منابع مشابه
Isolation of mouse lung dendritic cells.
Lung dendritic cells (DC) play a fundamental role in sensing invading pathogens (1,2) as well as in the control of tolerogenic responses (3) in the respiratory tract. At least three main subsets of lung dendritic cells have been described in mice: conventional DC (cDC) (4), plasmacytoid DC (pDC) (5) and the IFN-producing killer DC (IKDC) (6,7). The cDC subset is the most prominent DC subset in ...
متن کاملIsolation and Phenotyping of Normal Mouse Liver Dendritic Cells by an Improved Method
Introduction Dendritic cells (DCs) are bone marrow-derived cells, which migrate to lymphoid and non-lymphoid organs via blood. Liver DCs are believed to play an important role in the regulation of hepatic allograft acceptance. However, because of inherent difficulties in isolating adequate numbers of DCs from liver, limited information is available on the phenotype and functions of liver DCs. ...
متن کاملIsolation of mouse spleen dendritic cells.
It is now over 20 years since dendritic cells (DC) were first identified in and isolated from the spleens of mice (1,2) and they continue to be a much-studied population. Only a small proportion of spleen cells are DC, but the large size of the organ means that useful numbers of DC can still be purified. In recent years the ability to grow cells with the phenotypic and functional properties of ...
متن کاملIsolation, phenotype, and allostimulatory activity of mouse liver dendritic cells.
Donor liver-derived dendritic cells (DC) have recently been identified within various lymphoid and nonlymphoid tissues of organ allograft recipients, including nonimmunosuppressed mice transplanted with and permanently accepting major histocompatibility complex (MHC)-disparate hepatic allografts. These findings have raised questions about the basis of the tolerogenicity of the liver--and, in pa...
متن کاملIsolation of dendritic cells.
This unit presents two methods for preparing dendritic cells (DCs), a highly specialized type of antigen-presenting cell (APC). The first method involves the isolation of DCs from mouse spleen, resulting in a cell population that is highly enriched in accessory cell and APC function. A support protocol for collagenase digestion of splenocyte suspensions is described to increase the yield of den...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2011
ISSN: 1940-087X
DOI: 10.3791/3563